目的 建立测定麦冬药材中3个具有代表性的黄酮成分,甲基麦冬高黄酮A、麦冬甲基黄烷酮A和甲基麦冬二氢高异黄酮B的高效液相色谱法。方法 采用HPLC-DAD,色谱柱为Comatex C18柱(4.6 mm×250 mm,5 μm),流动相为乙腈-水(58∶42),流速1.0 mL·min-1,柱温30 ℃,检测波长296 nm,进样量20 μL。结果 甲基麦冬高黄酮A、麦冬甲基黄烷酮A和甲基麦冬二氢高异黄酮B 3种成分的线性方程分别为Y=493 321ρ+31 262(r=0.999 9)、Y=605 744ρ+40 941(r=0.999 9)、Y=586 672ρ+39 657(r=0.999 9);平均回收率分别为100.59%(RSD=1.51%)、99.27%(RSD=1.28%)、100.04%(RSD=1.33%)。结论 建立的麦冬3种黄酮含量测定方法准确灵敏、重复性好,可应用于麦冬药材质量评价中。
Abstract
OBJECTIVE To establish an HPLC method for simultaneous determination of methylophiopogonone A, methylophiopogonanone A, and methylophiopogonanone B. METHODS Comatex C18 column (4.6 mm×250 mm, 5 μm)was used for the HPLC analysis. The mobile phase consisted of acetonitrile and water (58∶42) and was eluted at the flow rate of 1 mL·min-1. The column temperature was maitained at 30 ℃. The detection wavelength was set at 280 nm. The injection volume was 15.0 μL. RESULTS The linear regression equations of methylophiopogonone A, methylophiopogonanone A and methylophiopogonanone B were Y=493 321ρ+31 262(r=0.999 9), Y=605 744ρ+40 941(r=0.999 9), and Y=586 672ρ+39 657(r=0.999 9), respectively. The average recovery rates of the three flavones respectively were 100.59%(RSD=1.51%), 99.27%(RSD=1.28%), and 100.04%(RSD=1.33%). CONCLUSION The established method for simultaneous determination of flavone constituents in Ophiopogonis Radix is accurate and sensitive, with good repeatability. It can be applied to the quality evaluation of Ophiopogonis Radix.
关键词
麦冬 /
黄酮 /
高效液相色谱法 /
含量
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Key words
Ophiopogonis Radix /
flavone /
HPLC /
content
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中图分类号：
R284
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参考文献
[1] Ch. P (2010) Vol Ⅰ(中国药典2010年版. 一部)[S]. 2010:144.
[2] SUN Z G, CHENG D L, ZHONG X B, et al. Current situation of protection of Fucheng and Xiang Ophiopog on japonicas, crude drugs and national products of geographical indications[J]. Guizhou Agric Sci(贵州农业科学),2010, 38(1):57-59.
[3] ZHANG Y P, CHEN J Z, AO Z H, et al. Research progress of active constituent in Ophiopogonis Radix of different species, different producing areas, different parts of plant [J]. China Prac Med(中国实用医药), 2008, 3(10):191-193.
[4] CHEN X Y, DU W F, CAI B C, et al. Rapid determination of total saponins of Radix Ophiopogonis from Zhejiang Province by nearinfrared spec-troscopy [J]. Tradit Chin Drug Res Clin Pharmacol (中药新药与临床药理), 2013, 24(1):85-88.
[5] HAO Y, ZHANG X G, CHENG Y W, et al. A preliminary study of the variation of the flavonoids content of domestic high quality Ophiopogon japonicas [J]. J Anhui Agric Sci(安徽农业科学), 2012, 40(33):16123-16124, 16128.
[6] TANG L Q, LI C, LIU S, et al. Determination of pachyman in Radix Ophiogonis content by anthrone-sulphuric acid colrimetry [J]. Anhui Med Pharm J (安徽医药),2003, 7(1):39-40.
[7] HAN F M, LI Z, ZHANG L, et al. Comparative studies on HPLC fingerprinting of Radix Ophiopogis and Radix Liriopes [J]. Special Wild Economic Animal and Plant Research(特产研究), 2007, 29(1):20-22.
[8] LIN Y N, SHIDA Y, YUAN B, et al. Comparative studies on fingerprints of root of Ophiopogon japonicas cultivated in different areas[J]. J China Pharm Univ (中国药科大学学报), 2005, 36(6):538-542.
[9] YU J P, MA Y G, SHAO J F, et al. Quantitative determination of ophiopogonin D in Zhe Ophiopogon japonicus(Thunb) Ker-Gawl and Chuan Ophiopogon japonicus(Thunb) Ker-Gawl by HPLC-ELSD [J]. Tradit Chin Drug Res Clin Pharmacol(中药新药与临床药理), 2002, 13(4):253-255.
[10] HE L, JIN H, LIANG X L, et al. Determination of ophiopogonin D in the tuber root and fibrous root of Ophiopogon japonicas by HPLC [J]. West China J Pharm Sci (华西药学杂志), 2014, 29(5):583-584.
[11] LIU J L, FAN Q J, ZHENG S L, et al. Quantitative determination of 5 active ingredients in different harvest periods of Ligusticumchuanxiong by HPLC[J]. China J Chin Mat Med (中国中药杂志), 2014, 39(9):1650-1655.
[12] HE B, YANG S Y, ZHANG Y. HPLC Simultaneous determination of 10 active constituents in Yinhuang Buccal Tablets [J]. Chin J Pharm Anal (药物分析杂志),2012,32(10):1853-1857, 1881.
[13] TAN S, WEN X S. Simultaneous determination of 6 active components in Chrysanthemum morifolium by HPLC [J]. China J Chin Mat Med (中国中药杂志), 2011, 36(11):1474-1477.
[14] GENG Z P, ZHENG H J, ZHANG Y, et al. Simultaneous determination of 6 active components in Chrysanthemum morifolium by HPLC[J]. China J Chin Mat Med (中国中药杂志),2010, 35(19):2576-2580.
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脚注
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基金
国家科技部“重大新药创制”资助项目(2014ZX09304307-002);科技部科技惠民资助项目(2013GS510102);四川省中医管理局资助项目(2014F038);四川省科技厅科技支撑重点资助项目(2015SZ0033)
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